![]() ![]() Membrane-compatible – colored bands transfer to membranes for Western blottingĭye-stained proteins in 62.5mM Tris-H 3PO 4 (pH 7.5 at 25☌), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN 3 and 33% glycerol. Quality tested – each lot evaluated by SDS-PAGE and Western blottingīright reference bands – orange at 70 and 25kDa, and green at 10kDa Sharp bands – color-coded bands of similar intesity for easy visualization Ready-to-use – supplied in a loading buffer for direct loading on gels no need to boil Kaleidoscope Prestained Natural Standards are broad range and have individually colored proteins to allow instant orientation on SDS-PAGE gels and western blots. Size range – nine proteins spanning 10 to 250kDa PageRuler Plus Prestained Protein Ladder is ready to use: no heating, further dilution or addition of a reducing agent is required before loading onto a gel. These standards can be detected by fluorescence when excited at appropriate wavelengths. ![]() A blue chromophore is bound to all proteins, except proteins of two reference bands of 70kDa and 25kDa that are colored with an orange dye and one green reference band of 10kDa. Precision Plus Protein Kaleidoscope Standards are a mixture of ten multicolored recombinant proteins (10250 kD). This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. Thermo Scientific™ PageRuler Plus Prestained Protein Ladder is a mixture of nine (9) blue-, orange- and green-stained proteins (10 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting. ![]()
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